TISSUE BIOELECTRODE FOR ORGANIC-PHASE ENZYMATIC ASSAYS

被引：49
作者：
WANG, J
NASER, N
KWON, HS
CHO, MY
机构：
[1] NEW MEXICO STATE UNIV,DEPT CHEM ENGN,LAS CRUCES,NM 88003
[2] CHUNGBUK NATL UNIV,DEPT SCI EDUC,CHONGJU,SOUTH KOREA
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 264卷 / 01期
关键词：
BIOSENSORS; ENZYMATIC METHODS; PHENOLS; TISSUE BIOELECTRODE; HYDROGEN PEROXIDE;
D O I：
10.1016/0003-2670(92)85290-M
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The utility of tissue bioelectrodes to serve as effective organic-phase biosensors is demonstrated. The organic-phase operation enables a simple immobilization procedure, based on spreading a thin layer of the tissue onto a rough graphite disk. Mushroom, banana or horseradish root layers (rich with tyrosinase or horseradish peroxidase) were thus immobilized and employed for quantifying the corresponding phenolic and peroxide species in chloroform media. The thin tissue layer and its immediate proximity to the graphite surface results in a very fast and sensitive amperometric response (8-20 s for steady state, and a detection limit of 3 X 10(-5) M). The influence of numerous experimental variables is explored, and future prospects are discussed.
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页码：7 / 12
页数：6
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