STAPHYLOCOCCAL PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM - PURIFICATION AND PROTEIN SEQUENCING OF THE STAPHYLOCOCCUS-CARNOSUS HISTIDINE-CONTAINING PROTEIN, AND CLONING AND DNA SEQUENCING OF THE PTSH GENE

被引：27

作者：

EISERMANN, R ^{[1
]}

FISCHER, R ^{[1
]}

KESSLER, U ^{[1
]}

NEUBAUER, A ^{[1
]}

HENGSTENBERG, W ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,DEPT MICROBIOL,NDEF 06,W-4630 BOCHUM,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 197卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb15875.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The histidine-containing protein (HPr) of the bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) was isolated from Staphylococcus carnosus and purified to homogeneity. The protein sequence was determined by Edman degradation of peptides obtained by proteolytic digestion with proteases V8, trypsin and chemical cleavage with BrCN. Furthermore, immunological screening of a chromosomal S. carnosus DNA gene library in pUC19 vector enabled us to isolate S. carnosus HPr-expressing colonies. The nucleotide sequence of this ptsH gene and its flanking regions was determined by the dideoxy-chain-termination technique. Upstream, the 264-bp open reading frame of the ptsH gene is flanked by a putative S. carnosus promoter structure and a putative ptsI gene downstream suggesting that ptsH gene is the first gene in the PTS operon of S. carnosus. Comparison of the amino acid sequence of S. carnosus HPr with the HPr sequence of Staphylococcus aureus (derived from peptide sequencing) showed a high degree of similarity.

引用

页码：9 / 14

页数：6

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