A 2ND THYROGLOBULIN MESSENGER-RNA SPECIES (RTG-2) IN RAT THYROCYTES

被引：20

作者：

GRAVES, PN ^{[1
]}

DAVIES, TF ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT MED,DIV ENDOCRINOL,NEW YORK,NY 10029

来源：

MOLECULAR ENDOCRINOLOGY | 1990年 / 4卷 / 01期

关键词：

D O I：

10.1210/mend-4-1-155

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A 0.95-kilobase (kb) thyrocyte RNA, initially detected in our 1B-6 subclone of Fisher rat thyrocytes (FRTL-5) using an oligonucleotide probe complementary to the 5′ end of rat thyroglobulin (Tg) mRNA, was also detected in cultured thyrocytes of the Wistar rat (WRT cells) and in freshly isolated normal rat thyroid tissue. This transcript was thyroid specific and as abundant as the previously characterized 9.0-kb Tg mRNA in the cultured thyroid cells under the growth conditions employed. The smaller RNA (designated rTg-2 mRNA) was cytoplasmic, polyadenylated, and regulated by TSH. Preliminary characterization with several oligonucleotide probes showed that rTg-2 shared coding information present at the 5′, but not the 3′, end of the 9.0-kb Tg mRNA. Sequencing of the cloned rTg-2 cDNA showed that it was homologous to human and other higher vertebrate Tg cDNAs at its 5′ coding end, but contained additional nonhomologous coding and noncoding sequences at the 3′ end. The junction between the shared and unique sequences in rTg-2 mRNA occurred at the exon-5/intron-5 boundary in the Tg gene. This smaller transcript has not previously been reported and encodes elements known to be of structural and functional significance in the 330-kD Tg monomer. A putative polypeptide product from rTg-2 mRNA may play an important role in thyroid function and thyroid autoimmunity. © 1990 by The Endocrine Society.

引用

页码：155 / 161

页数：7

共 39 条

[1]

AMBESIIMPIOMBAT.FS, 1982, GROWTH CELLS HORMONA, P483

[2] STRUCTURAL ORGANIZATION OF THE 3' HALF OF THE RAT THYROGLOBULIN GENE [J].

AVVEDIMENTO, VE ;

MUSTI, AM ;

OBICI, S ;

COCOZZA, S ;

DILAURO, R .

NUCLEIC ACIDS RESEARCH, 1984, 12 (08) :3461-3472

[3] DIFFERENTIAL EXPRESSION OF THYROGLOBULIN GENE IN NORMAL AND TRANSFORMED THYROID-CELLS [J].

AVVEDIMENTO, VE ;

MONTICELLI, A ;

TRAMONTANO, D ;

POLISTINA, C ;

NITSCH, L ;

DILAURO, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 149 (03) :467-472

[4] SPECIFICITY OF OLIGO (DT)-CELLULOSE CHROMATOGRAPHY IN ISOLATION OF POLYADENYLATED RNA [J].

BANTLE, JA ;

MAXWELL, IH ;

HAHN, WE .

ANALYTICAL BIOCHEMISTRY, 1976, 72 (1-2) :413-427

[5] INSULIN STIMULATES CELL-GROWTH OF A NEW STRAIN OF DIFFERENTIATED RAT-THYROID CELLS [J].

BRANDI, ML ;

ROTELLA, CM ;

MAVILIA, C ;

FRANCESCHELLI, F ;

TANINI, A ;

TOCCAFONDI, R .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1987, 54 (01) :91-103

[6] OVALBUMIN GENE - EVIDENCE FOR A LEADER SEQUENCE IN MESSENGER-RNA AND DNA SEQUENCES AT EXON-INTRON BOUNDARIES [J].

BREATHNACH, R ;

BENOIST, C ;

OHARE, K ;

GANNON, F ;

CHAMBON, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (10) :4853-4857

[7] MOLECULAR-WEIGHT OF THYROGLOBULIN MESSENGER-RNA OF SHEEP THYROID-GLAND [J].

CHEBATH, J ;

CHABAUD, O ;

BERGELEFRANC, JL ;

CARTOUZOU, G ;

LISSITZKY, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 79 (01) :267-273

[8] CLONING THE FISHER RAT-THYROID CELL-LINE (FRTL-5) - VARIABILITY IN CLONAL GROWTH AND 3,'5'-CYCLIC ADENOSINE-MONOPHOSPHATE RESPONSE TO THYROTROPIN [J].

DAVIES, TF ;

YANG, C ;

PLATZER, M .

ENDOCRINOLOGY, 1987, 121 (01) :78-83

[9]

DILAURO R, 1985, EUR J BIOCHEM, V148, P7

[10]

DUNN JT, 1987, J BIOL CHEM, V262, P16948

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