PHOSPHORYLATION OF POLY(ADP-RIBOSE)POLYMERASE PROTEIN IN HUMAN PERIPHERAL LYMPHOCYTES STIMULATED WITH PHYTOHEMAGGLUTININ

被引：12
作者：
BAUER, PI
FARKAS, G
MIHALIK, R
KOPPER, L
KUN, E
FARAGO, A
机构：
[1] SEMMELWEIS UNIV MED, SCH MED, DEPT BIOCHEM 1, H-1444 BUDAPEST 8, HUNGARY
[2] SEMMELWEIS UNIV MED, SCH MED, DEPT BIOCHEM 2, H-1444 BUDAPEST 8, HUNGARY
[3] NATL INST HAEMATOL & BLOOD TRANSFUS, H-1113 BUDAPEST, HUNGARY
[4] SEMMELWEIS UNIV MED, SCH MED, INST PATHOL & EXPTL CANC RES, H-1085 BUDAPEST, HUNGARY
[5] SAN FRANCISCO STATE UNIV, ROMBERG TIBURON CTR, ENVIRONM TOXICOL & CHEM LAB, TIBURON, CA 94920 USA
[6] SAN FRANCISCO STATE UNIV, ROMBERG TIBURON CTR, OCTAMER RES FDN, TIBURON, CA 94920 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1223卷 / 02期
基金：
匈牙利科学研究基金会;
关键词：
POLY(ADP-RIBOSE)POLYMERASE; PROTEIN KINASE; PHORBOL MYRISTYL ACETATE; PHYTOHEMAGGLUTININ; SIGNAL TRANSDUCTION; PHOSPHORYLATION; (LYMPHOCYTE);
D O I：
10.1016/0167-4889(94)90231-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Intracellular phosphorylation of poly(ADP-ribose)polymerase was assayed in streptolysin-O-permeabilized human lymphocytes. Whereas P-32 incorporation from [gamma-P-32]ATP into immunoprecipitated enzyme protein was undetectable in resting cells, significant phosphorylation of this enzyme was observed in lymphocytes treated with phytohemagglutinin. The phosphorylation of poly(ADP-ribose)polymerase in permeabilized cells was not stimulated by phorbol ester, while phorbol-induced phosphorylation of other proteins and of a specific oligopeptide substrate of protein kinase C was observed. However, the specific inhibitory pseudosubstrate peptide of protein kinase C blocked the phosphorylation of poly(PLDP-ribose)polymerase induced by phytohemagglutinin. Therefore, a potential role of a member of the protein kinase C family in the phytohemagglutinin stimulated intracellular phosphorylation of poly(ADP-ribose)polymerase is conceivable.
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页码：234 / 239
页数：6
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