CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS

被引：14

作者：

CHARLES, TC ^{[1
]}

DOTY, SL ^{[1
]}

NESTER, EW ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT MICROBIOL, SEATTLE, WA 98195 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 11期

关键词：

D O I：

10.1128/AEM.60.11.4192-4194.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

引用

页码：4192 / 4194

页数：3

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