CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS

被引：14

作者：

CHARLES, TC ^{[1
]}

DOTY, SL ^{[1
]}

NESTER, EW ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT MICROBIOL, SEATTLE, WA 98195 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 11期

关键词：

D O I：

10.1128/AEM.60.11.4192-4194.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

引用

页码：4192 / 4194

页数：3

共 22 条

[1] SUGAR-MEDIATED INDUCTION OF AGROBACTERIUM-TUMEFACIENS VIRULENCE GENES - STRUCTURAL SPECIFICITY AND ACTIVITIES OF MONOSACCHARIDES [J].

ANKENBAUER, RG ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6442-6446

[2] SPECIFICITY OF AGROBACTERIUM-MEDIATED DELIVERY OF MAIZE STREAK VIRUS-DNA TO MEMBERS OF THE GRAMINEAE [J].

BOULTON, MI ;

BUCHHOLZ, WG ;

MARKS, MS ;

MARKHAM, PG ;

DAVIES, JW .

PLANT MOLECULAR BIOLOGY, 1989, 12 (01) :31-40

[3]

BOULTON MI, 1992, ADV MOL GENETICS PLA, P73

[4] CORRELATIVE ASSOCIATION BETWEEN RESIDENT PLASMIDS AND THE HOST CHROMOSOME IN A DIVERSE AGROBACTERIUM SOIL POPULATION [J].

BOUZAR, H ;

OUADAH, D ;

KRIMI, Z ;

JONES, JB ;

TROVATO, M ;

PETIT, A ;

DESSAUX, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) :1310-1317

[5]

CANGELOSI GA, 1991, METHOD ENZYMOL, V204, P384

[6] SUGARS INDUCE THE AGROBACTERIUM VIRULENCE GENES THROUGH A PERIPLASMIC BINDING-PROTEIN AND A TRANSMEMBRANE SIGNAL PROTEIN [J].

CANGELOSI, GA ;

ANKENBAUER, RG ;

NESTER, EW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6708-6712

[7] A CHROMOSOMALLY ENCODED 2-COMPONENT SENSORY TRANSDUCTION SYSTEM IS REQUIRED FOR VIRULENCE OF AGROBACTERIUM-TUMEFACIENS [J].

CHARLES, TC ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1993, 175 (20) :6614-6625

[8] AGROBACTERIUM-TUMEFACIENS DNA AND PS8 BACTERIOPHAGE DNA NOT DETECTED IN CROWN GALL TUMORS [J].

CHILTON, MD ;

CURRIER, TC ;

FARRAND, SK ;

BENDICH, AJ ;

GORDON, MP ;

NESTER, EW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (09) :3672-3676

[9] DESIGN AND DEVELOPMENT OF AMPLIFIABLE BROAD-HOST-RANGE CLONING VECTORS - ANALYSIS OF THE VIR REGION OF AGROBACTERIUM-TUMEFACIENS PLASMID PTIC58 [J].

CLOSE, TJ ;

ZAITLIN, D ;

KADO, CI .

PLASMID, 1984, 12 (02) :111-118

[10] THE CHROMOSOMAL VIRULENCE GENE, CHVE, OF AGROBACTERIUM-TUMEFACIENS IS REGULATED BY A LYSR FAMILY MEMBER [J].

DOTY, SL ;

CHANG, M ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1993, 175 (24) :7880-7886

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