CRYSTALLIZATION OF BACILLUS-SUBTILIS TRYPTOPHANYL-TRANSFER RNA-SYNTHETASE

被引：2
|
作者：
ALA, P [1 ]
XUE, H [1 ]
LEUNG, L [1 ]
XUE, YQ [1 ]
WONG, JTF [1 ]
YANG, DSC [1 ]
机构：
[1] UNIV TORONTO,DEPT BIOCHEM,TORONTO M5S 1A1,ONTARIO,CANADA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 03期
关键词：
CRYSTALLIZATION; TRYPTOPHANYL-TRANSFER RNA SYNTHETASE; CRYSTALLOGRAPHY; AMINOACYL-TRANSFER RNA SYNTHETASE;
D O I：
10.1006/jmbi.1993.1223
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tryptophanyl-tRNA synthetase from Bacillus subtilis was overexpressed in Escherichia coli and was purified using column chromatography on DEAE-Sephacel and hydroxyapatite columns. Single crystals of the synthetase were grown by vapor diffusion at 4°C from pH 5.5 solutions of polyethylene glycol 8000 containing magnesium ATP and L-tryptophan. The crystals diffracted to about 4.0 Å resolution at -150°C and appeared to belong to the orthorhombic space group P21212 with unit cell dimensions: a = 143.6 Å, b = 111.6 Å, c = 50.6 Å with one dimer in the asymmetric unit. © 1993 Academic Press, Inc.
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收藏
页码：1089 / 1090
页数：2
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