TEMPERATURE-PROGRAMMED CAPILLARY AFFINITY GEL-ELECTROPHORESIS FOR THE SENSITIVE BASE-SPECIFIC SEPARATION OF OLIGODEOXYNUCLEOTIDES

被引:30
作者
BABA, Y [1 ]
TSUHAKO, M [1 ]
SAWA, T [1 ]
AKASHI, M [1 ]
机构
[1] KAGOSHIMA UNIV,FAC ENGN,DEPT APPL CHEM & CHEM ENGN,KAGOSHIMA 890,JAPAN
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 632卷 / 1-2期
关键词
D O I
10.1016/0021-9673(93)80036-8
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High-performance base-specific separations of oligodeoxynucleotides were performed by temperature-programmed capillary affinity gel electrophoresis (CAGE), in which poly(9-vinylacenine) (PVAd) was utilized as an affinity ligand. The migration behaviour of oligodeoxynucleotides was investigated at different capillary temperatures. The migration time and resolution of oligodeoxyadenylic acids, which do not interact with PVAd, decrease with increase in temperature as in capillary gel electrophoretic separation. The migration behaviour of oligothymidylic acids, which interact with PVAd, is manipulated by varying the capillary temperature, which leads to changes in the dissociation process of specific hydrogen bonding between oligothymidylic acids and PVAd. The implementation of temperature-programmed CAGE was illustrated by the selective and sensitive base recognition of oligodeoxynucleotides with efficiencies as high as several times 10(6) plates per metre.
引用
收藏
页码:137 / 142
页数:6
相关论文
共 16 条
[1]   AFFINITY CHROMATOGRAPHIC-SEPARATION .2. NOVEL STATIONARY PHASES FOR AFFINITY-CHROMATOGRAPHY - NUCLEOBASE-SELECTIVE RECOGNITION OF NUCLEOSIDES AND NUCLEOTIDES ON POLY(9-VINYLADENINE)-SUPPORTED SILICA-GEL [J].
AKASHI, M ;
YAMAGUCHI, M ;
MIYATA, H ;
HAYASHI, M ;
YASHIMA, E ;
MIYAUCHI, N .
CHEMISTRY LETTERS, 1988, (07) :1093-1096
[2]  
AKASHI M, 1989, J BIOACT COMPAT POLY, V4, P124
[3]  
AKASHI M, 1992, J HIGH RES CHROMATOG, V15, P625
[4]  
[Anonymous], UNPUB
[5]   SPECIFIC BASE RECOGNITION OF OLIGODEOXYNUCLEOTIDES BY CAPILLARY AFFINITY GEL-ELECTROPHORESIS USING POLYACRYLAMIDE POLY(9-VINYLADENINE) CONJUGATED GEL [J].
BABA, Y ;
TSUHAKO, M ;
SAWA, T ;
AKASHI, M ;
YASHIMA, E .
ANALYTICAL CHEMISTRY, 1992, 64 (17) :1920-1925
[6]   A SIMPLE METHOD FOR THE PREPARATION OF POLYACRYLAMIDE-GEL FILLED CAPILLARIES FOR HIGH-PERFORMANCE SEPARATION OF POLYNUCLEOTIDES BY USING CAPILLARY GEL-ELECTROPHORESIS [J].
BABA, Y ;
MATSUURA, T ;
WAKAMOTO, K ;
TSUHAKO, M .
CHEMISTRY LETTERS, 1991, (03) :371-374
[7]   COMPARISON OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CAPILLARY GEL-ELECTROPHORESIS IN SINGLE-BASE RESOLUTION OF POLYNUCLEOTIDES [J].
BABA, Y ;
MATSUURA, T ;
WAKAMOTO, K ;
TSUHAKO, M .
JOURNAL OF CHROMATOGRAPHY, 1991, 558 (01) :273-284
[8]   GEL-FILLED CAPILLARIES FOR NUCLEIC-ACID SEPARATIONS IN CAPILLARY ELECTROPHORESIS [J].
BABA, Y ;
TSUHAKO, M .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1992, 11 (08) :280-287
[9]   PREPARATION OF POLYACRYLAMIDE-GEL FILLED CAPILLARIES FOR ULTRAHIGH RESOLUTION OF POLYNUCLEOTIDES BY CAPILLARY GEL-ELECTROPHORESIS [J].
BABA, Y ;
MATSUURA, T ;
WAKAMOTO, K ;
MORITA, Y ;
NISHITSU, Y ;
TSUHAKO, M .
ANALYTICAL CHEMISTRY, 1992, 64 (11) :1221-1225
[10]   HIGH-PERFORMANCE AFFINITY-CHROMATOGRAPHY OF DNA [J].
GOSS, TA ;
BARD, M ;
JARRETT, HW .
JOURNAL OF CHROMATOGRAPHY, 1990, 508 (02) :279-287