CLONING AND SEQUENCE-ANALYSIS OF MEMBRANE-BOUND ALKALINE-PHOSPHATASE CDNA OF THE SILKWORM, BOMBYX-MORI

被引：25
|
作者：
ITOH, M [1 ]
TAKEDA, S [1 ]
YAMAMOTO, H [1 ]
IZUMI, S [1 ]
TOMINO, S [1 ]
EGUCHI, M [1 ]
机构：
[1] TOKYO METROPOLITAN UNIV,DEPT BIOL,TOKYO 158,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1129卷 / 01期
关键词：
MEMBRANE-BOUND ALKALINE PHOSPHATASE; CDNA SEQUENCE; PROTEIN EVOLUTION; SILKWORM; (BOMBYX-MORI);
D O I：
10.1016/0167-4781(91)90229-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The nucleotide sequence (1974 bp) of cDNA coding for membrane-bound alkaline phosphatases (m-ALP) of Bombyx mori was isolated. The cDNA clone contained an open reading frame encoding a polypeptide (547 amino acids), which contains a hydrophobic signal peptide of 36 amino acids and the mature protein of 511 amino acids (M(r) = 56 163). We found a highly hydrophobic domain presumed to be a membrane anchoring region at the C-terminus. Comparing analysis between Bombyx m-ALP and mammalian and Escherichia coli ALPs suggested an evolutionary relationship of sharing a common ancestral gene.
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页码：135 / 138
页数：4
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