CONSERVATION OF BINDING-SITE SPECIFICITY OF 3 YEAST DNA-BINDING PROTEINS

被引：1
|
作者：
PRETORIUS, GHJ
MULLER, HE
机构：
[1] Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, 9300
来源：
FEBS LETTERS | 1992年 / 298卷 / 2-3期
关键词：
TRANSCRIPTION FACTOR; PROTEIN-DNA INTERACTION; MOBILITY SHIFT ASSAY;
D O I：
10.1016/0014-5793(92)80057-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sequence specific binding of protein extracts from 13 different yeast species to three oligonucleotide probes and two points mutants derived from Saccharomyces cerevisiae DNA binding proteins were tested using mobility shift assays. The probes were high affinity binding sites for GRF1/RAP1/ABF1 and CP1/CPF1. Most yeasts in the genus Saccharomyces showed specific binding to all three probes and also displayed similar sequence requirements when challenged by molar excesses of mutant probes. The affinities for the probes varied amongst the other yeasts tested, but in general, CPF1 binding activity was the most widespread, while the other two were more limited.
引用
收藏
页码：203 / 205
页数：3
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