LIQUID-CHROMATOGRAPHY WITH ELECTROCATALYTIC DETECTION OF CYSTEINE, N-ACETYLCYSTEINE AND GLUTATHIONE BY A PRUSSIAN BLUE FILM-MODIFIED ELECTRODE

被引：77

作者：

HOU, WY ^{[1
]}

WANG, EK ^{[1
]}

机构：

[1] CHINESE ACAD SCI, CHANGCHUN INST APPL CHEM, ELECTROANALYT CHEM LAB, CHANGCHUN 130022, PEOPLES R CHINA

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1991年 / 316卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(91)87043-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chemically modified electrodes prepared by adsorbing prussian blue on a glassy carbon electrode are shown to catalyse the electro-oxidation of cysteine, N-acetylcysteine and glutathione in acidic media. The catalytic response is evaluated with respect to the potential scan rate, the solution pH, the concentration dependence, and other variables. Covering the electrode with Nafion(R) film improved the stability and reproducibility in liquid chromatography with electrochemical detection to the extent that repetitive sample injections produced relative standard deviations of less than 5% over several hours of operation. The limit of detection was 4 pmol for cysteine, 33 pmol for glutathione and 61 pmol for N-acetylcysteine.

引用

页码：155 / 163

页数：9

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