THE WRINKLED-SEED CHARACTER OF PEA DESCRIBED BY MENDEL IS CAUSED BY A TRANSPOSON-LIKE INSERTION IN A GENE ENCODING STARCH-BRANCHING ENZYME

被引：332

作者：

BHATTACHARYYA, MK ^{[1
]}

SMITH, AM ^{[1
]}

ELLIS, THN ^{[1
]}

HEDLEY, C ^{[1
]}

MARTIN, C ^{[1
]}

机构：

[1] AFRC,INST PLANT SCI RES,NORWICH NR4 7UH,ENGLAND

来源：

CELL | 1990年 / 60卷 / 01期

关键词：

D O I：

10.1016/0092-8674(90)90721-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the cloning of the r (rugosus) locus of pea (Pisum sativum L.), which determines whether the seed is round or wrinkled. Wrinkled (rr) seeds lack one isoform of starch-branching enzyme (SBEI), present in round (RR or Rr) seeds. A major polymorphism in the SBEI gene between near-isogenic RR and rr lines shows 100% cosegregation with the r locus, establishing that the SBEI gene is at the r locus. An aberrant transcript for SBEI is produced in rr embryos. In rr lines the SBEI gene is interrupted by a 0.8 kb insertion that is very similar to the Ac/Ds family of transposable elements from maize. Failure to produce SBEI has complex metabolic consequences on starch, lipid, and protein biosynthesis in the seed. © 1990.

引用

页码：115 / 122

页数：8

共 53 条

[1] AN ANALYSIS OF SEED DEVELOPMENT IN PISUM-SATIVUM .4. COTYLEDON CELL-POPULATIONS INVIVO AND INVITRO [J].