OVERLAPPING SEQUENCES OF KLEBSIELLA-PNEUMONIAE-NIF DNA CLONED AND CHARACTERIZED

被引：154

作者：

CANNON, FC ^{[1
]}

RIEDEL, GE ^{[1
]}

AUSUBEL, FM ^{[1
]}

机构：

[1] HARVARD UNIV,DEPT BIOL,CAMBRIDGE,MA 02138

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 174卷 / 01期

关键词：

D O I：

10.1007/BF00433306

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A HindIII (17.0 kb) and an EcoRl restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonculease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb. © 1979 Springer-Verlag.

引用

页码：59 / 66

页数：8

共 23 条

[1]

AUSUBEL F, 1977, GENETIC ENGINEERING, P111

[2]

BOYER HW, 1977, RECOMBINANT MOL IMPA, P9

[3]

CANNON F, 1978, GENETIC ENGENEERING, P181

[4] RECOMBINANT PLASMID THAT CARRIES PART OF NITROGEN-FIXATION (NIF) GENE CLUSTER OF KLEBSIELLA-PNEUMONIAE [J].