Embryogenic callus was formed on the hypocotyl of immature zygotic embryos under the influence of 2,4-dichlorophenoxyacetic acid (2,4-D) in liquid as well as in agar medium. Germination was induced by chilling but not by desiccation treatments. Epicotyl dormancy was overcome by placing the embryos with elongated radicles on medium with 6-benzyl-adenine (BA). Normally developed plantlets were transferred to soil, and acclimated in a greenhouse.
机构:
Instituto Madrileño de Investigación Agraria, Alcalá de Henares, Madrid 28800Instituto Madrileño de Investigación Agraria, Alcalá de Henares, Madrid 28800
García-Martín G.
González-Benito M.E.
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Departamento de Biología Vegetal, Escuela Universitaria de Ingeniería Técnica Agrícola, Ciudad UniversitariaInstituto Madrileño de Investigación Agraria, Alcalá de Henares, Madrid 28800
González-Benito M.E.
Manzanera J.A.
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机构:
Departamento de Silvopascicultura, Universidad Politécnica de Madrid, Ciudad UniversitariaInstituto Madrileño de Investigación Agraria, Alcalá de Henares, Madrid 28800
机构:
Chinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Yu, Xinran
Zhang, Wan
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Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Zhang, Wan
Zhu, Haiyang
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Chinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Zhu, Haiyang
Wang, Yijun
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Chinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Wang, Yijun
Hu, Chenrong
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机构:
Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Hu, Chenrong
Yang, Yun
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机构:
Huanghuai Univ, Coll Biol & Food Engn, Zhumadian 463000, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China
Yang, Yun
Zhu, Jingle
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机构:
Chinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R ChinaChinese Acad Forestry, Res Inst Nontimber Forestry, Zhengzhou 450003, Peoples R China