INHIBITION OF HELICOBACTER-PYLORI UREASE BY OMEPRAZOLE

被引：54

作者：

BUGNOLI, M ^{[1
]}

BAYELI, PF ^{[1
]}

RAPPUOLI, R ^{[1
]}

PENNATINI, C ^{[1
]}

FIGURA, N ^{[1
]}

CRABTREE, JE ^{[1
]}

机构：

[1] ST JAMES UNIV HOSP,DEPT CLIN MED,LEEDS LS9 7TF,W YORKSHIRE,ENGLAND

来源：

EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY | 1993年 / 5卷 / 09期

关键词：

HELICOBACTER-PYLORI; UREASE; OMEPRAZOLE; PROTON PUMP INHIBITORS;

D O I：

10.1097/00042737-199309000-00003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objectives: To investigate the effect of the proton pump inhibitor omeprazole on the function of purified urease enzyme of Helicobacter pylori and to examine the in vitro activities of omeprazole against urease-positive and -negative Helicobacter strains. Methods: Urease was purified chromatographically from H. pylori. The effect of omeprazole on urease enzyme activity was assayed spectrophotometrically after incubation with urea substrate, phenol red indicator and 3 mmol/l phosphate buffer. Minimal inhibitory concentrations of omeprazole against H. pylori and other Helicobacter species were determined using an agar dilution assay. Results: Omeprazole was found to inhibit the enzyme activity of purified H. pylori urease in a dose-dependent manner. In vitro growth of both urease-positive and mutant urease-negative H. pylori strains was equally inhibited by omeprazole. Conclusions: Whilst omeprazole competitively inhibits the urease enzyme in vitro, urease enzyme inhibition does not account for the in vitro susceptibility of H. pylori to proton pump inhibitors. Urease inhibition by omeprazole in vivo, however, could increase the effectiveness of antibiotics. It may also affect the reliability of the urease-based tests used to assess H. pylori status.

引用

页码：683 / 685

页数：3

共 13 条

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