CLONING AND EXPRESSION OF THE MOUSE HOMOLOG OF THE HUMAN ALPHA-2-C2 ADRENERGIC-RECEPTOR

Three subtypes of α2 adrenergic receptors have been identified in the human and rat. The subtype located on human chromosome 2 (α2-C2) is unique in that it is expressed mainly in the peripheral tissues and lacks sites for N-linked glycosylation. We isolated the gene encoding the mouse homolog of the human α2-C2 adrenergic receptor (Mα2-2H). The deduced amino acid sequence of the Mα2-2H shows 82% and 96% identity to the human α2-C2 and the rat RNGα2 adrenergic receptors, respectively. Southern blot analysis demonstrated that the Mα2-2H was encoded by a single copy gene and was distinct from the mouse homologs of the α2-C4 and α2-C10 adrenergic receptors. When expressed in COS-7 cells, the Mα2-2H exhibited a pharmacological profile similar to the human α2-C2 and rat RNGα2 receptors. © 1992 Academic Press, Inc.