TRANSFORMATION AND EXPRESSION OF A STAPHYLOCOCCAL PLASMID IN ESCHERICHIA-COLI

被引：1

作者：

SAHA, D

BAL, M

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 109卷 / 2-3期

关键词：

PLASMID; STAPHYLOCOCCUS-AUREUS; TRANSFORMATION; ESCHERICHIA-COLI;

D O I：

10.1016/0378-1097(93)90033-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiple antibiotic-resistant Staphvlococcus aureus, was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one EcoRI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recA- E. coli at a frequency of 1.2 X 10(4) transformants/mug of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus.

引用

页码：279 / 282

页数：4

共 11 条

[1]

Acar JF, 1980, ANTIBIOTICS LAB MED, P24

[2] GENOME CONSTRUCTION BETWEEN BACTERIAL SPECIES INVITRO - REPLICATION AND EXPRESSION OF STAPHYLOCOCCUS PLASMID GENES IN ESCHERICHIA-COLI [J].