QUANTITATION OF ADIPOSE CONVERSION AND TRIGLYCERIDES BY STAINING INTRACYTOPLASMIC LIPIDS WITH OIL RED-O

被引：966

作者：

RAMIREZZACARIAS, JL ^{[1
]}

CASTROMUNOZLEDO, F ^{[1
]}

KURIHARCUCH, W ^{[1
]}

机构：

[1] INST POLITECN NACL, DEPT CELL BIOL, CTR INVEST & ESTUDIOS AVANZADOS, APDO POSTAL 14-740, MEXICO CITY 07000, MEXICO

来源：

HISTOCHEMISTRY | 1992年 / 97卷 / 06期

关键词：

D O I：

10.1007/BF00316069

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cultured 3T3-F442A cells differentiate into adipocytes and accumulate lipid droplets in the cytoplasm. When fat cells are stained with Oil red O, the degree of staining seems to be proportional to the extent of cell differentiation. We report here a fast and simple method to quantitate the extent of adipose conversion by staining the accumulated lipid with Oil red O and determining the amount of extracted dye at 510 nm. The results show that Oil red O specifically stains triglycerides and cholesteryl oleate but no other lipids. This technique is a valuable tool for processing large numbers of cell cultures or samples in which adipose differentiation and/or accumulated triglycerides is to be quantitated.

引用

页码：493 / 497

页数：5

共 25 条

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