MOLECULAR-SPECIES ANALYSIS OF 1,2-DIACYLGLYCEROLS AND PHOSPHATIDIC-ACID FORMED DURING BOMBESIN STIMULATION OF SWISS 3T3 CELLS

被引：18
|
作者：
DIVECHA, N
LANDER, DJ
SCOTT, TW
IRVINE, RF
机构：
[1] AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Cambridge, Babraham Hall
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1093卷 / 2-3期
关键词：
MOLECULAR SPECIES; 1,2-DIACYLGLYCEROL; PHOSPHATIDIC ACID; MITOGEN STIMULATION; (SWISS 3T3 CELL); PROTEIN-KINASE-C; PHOSPHATIDYLCHOLINE HYDROLYSIS; GROWTH-FACTOR; DIACYLGLYCEROL METABOLISM; DIFFERENT MECHANISMS; INOSITOL PHOSPHATES; ENDOTHELIAL-CELLS; PANCREATIC-ISLETS; ACTIVATION; GENERATION;
D O I：
10.1016/0167-4889(91)90121-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Swiss 3T3 cells were labelled with [H-3]glycerol and stimulated with bombesin over a time course of 20 min. The individual 1,2-diacylglycerols produced were quantified by acetylation followed by analysis by HPLC and argentation chromatography. The major phospholipids and phosphatidic acid were acetolysed and then analysed in the same manner. The data show that even at an early time of stimulation (30 s), stimulated diacylglycerol formation comes from at least two sources - phosphoinositides and phosphatidylcholine.
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页码：184 / 188
页数：5
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