A MEMBER OF THE CLPB FAMILY OF STRESS PROTEINS IS EXPRESSED DURING HEAT-SHOCK IN LEISHMANIA SPP

被引：51

作者：

HUBEL, A ^{[1
]}

BRANDAU, S ^{[1
]}

DRESEL, A ^{[1
]}

CLOS, J ^{[1
]}

机构：

[1] BERNHARD NOCHT INST TROP MED, D-20359 HAMBURG, GERMANY

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1995年 / 70卷 / 1-2期

关键词：

HEAT SHOCK; LEISHMANIA; GENE REGULATION;

D O I：

10.1016/0166-6851(95)00012-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified and isolated the Leishmania major homologue to the bacterial ClpB gene and to the yeast Hsp104 gene. ClpB in Leishmania major is a single-copy gene and encodes a low-abundance mRNA which is induced several-fold during a heat stress. We raised antibodies against the product of the recombinant gene and show that the leishmanial ClpB encodes a predominantly cytoplasmic protein of approx. 100 kDa which is detectable in Leishmania promastigotes of various species after exposure to elevated temperatures. We, therefore, term this protein Hsp100.

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页码：107 / 118

页数：12

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