SOLUBILIZATION AND CHARACTERIZATION OF [H-3] IMIPRAMINE AND [H-3] PAROXETINE BINDING-SITES FROM CALF STRIATUM

被引：4

作者：

ROTONDO, A ^{[1
]}

GIANNACCINI, G ^{[1
]}

QUATTRONE, C ^{[1
]}

MARAZZITI, D ^{[1
]}

MARTINI, C ^{[1
]}

CASSANO, GB ^{[1
]}

LUCACCHINI, A ^{[1
]}

机构：

[1] UNIV PISA,IST POLICATTEDRA DISCIPLINE BIOL,I-56100 PISA,ITALY

来源：

NEUROCHEMICAL RESEARCH | 1994年 / 19卷 / 10期

关键词：

SEROTONIN; NEURONAL SEROTONIN TRANSPORTER; CALF STRIATUM; H-3] IMIPRAMINE BINDING; H-3] PAROXETINE BINDING; PURIFICATION;

D O I：

10.1007/BF01006821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The serotonin (5-HT) transporter from calf striatum cerebral membranes was solubilized with digitonin and characterized by gel exclusion chromatography. [H-3]Imipramine and [H-3]paroxetine were utilized as markers for labeling it. H-3-imipramine labels a high- and a low-affinity site on striatum membranes, whereas it binds to a single high-affinity site on the solubilized fraction. [H-3]Paroxetine binds with the same affinity to a single site on both membranes and solubilized preparations. After gel exclusion chromatography of the solubilizate both [H-3]imipramine rind [H-3]paroxetine bind on an identical fraction of 205 kDa molecular weight, with a similar maximum number of binding sites (Bmax). Our results suggest that both H-3-imipramine and [H-3]paroxetine bind to a common site on the 5-HT transporter.

引用

页码：1295 / 1300

页数：6

共 25 条

[11] DIFFERENCES BETWEEN SODIUM-DEPENDENT AND DESIPRAMINE-DEFINED [H-3]IMIPRAMINE BINDING IN INTACT HUMAN-PLATELETS [J].

HRDINA, PD .

BIOLOGICAL PSYCHIATRY, 1989, 25 (05) :576-584

[12]

LAUNAY JM, 1992, J BIOL CHEM, V267, P11344

[13] PRIMARY STRUCTURE OF THE HUMAN PLATELET SEROTONIN UPTAKE SITE - IDENTITY WITH THE BRAIN-SEROTONIN TRANSPORTER [J].

LESCH, KP ;

WOLOZIN, BL ;

MURPHY, DL ;

RIEDERER, P .

JOURNAL OF NEUROCHEMISTRY, 1993, 60 (06) :2319-2322

[14] ISOLATION OF A CDNA-ENCODING THE HUMAN BRAIN-SEROTONIN TRANSPORTER [J].

LESCH, KP ;

WOLOZIN, BL ;

ESTLER, HC ;

MURPHY, DL ;

RIEDERER, P .

JOURNAL OF NEURAL TRANSMISSION-GENERAL SECTION, 1993, 91 (01) :67-72

[15]

MARCUSSON JO, 1986, MOL PHARMACOL, V30, P121

[16] BINDING OF SOME ANTIDEPRESSANTS TO THE 5-HYDROXYTRYPTAMINE TRANSPORTER IN BRAIN AND PLATELETS [J].

MARCUSSON, JO ;

ROSS, SB .

PSYCHOPHARMACOLOGY, 1990, 102 (02) :145-155

[17]

MCPHERSON GA, 1985, KINETIC EBDA LIGAND

[18] HIGH-AFFINITY BINDING OF [H-3]LABELED PAROXETINE AND [H-3]IMIPRAMINE TO HUMAN-PLATELET MEMBRANES [J].

MELLERUP, ET ;

PLENGE, P ;

ENGELSTOFT, M .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1983, 96 (3-4) :303-309

[19] ALLOSTERIC INTERACTION BETWEEN THE SITE LABELED BY [H-3] IMIPRAMINE AND THE SEROTONIN TRANSPORTER IN HUMAN-PLATELETS [J].

MEYERSON, LR ;

IENI, JR ;

WENNOGLE, LP .

JOURNAL OF NEUROCHEMISTRY, 1987, 48 (02) :560-565

[20]

PETERSON GL, 1977, ANAL BIOCHEM, V83, P356

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