SOLUBILIZATION AND CHARACTERIZATION OF [H-3] IMIPRAMINE AND [H-3] PAROXETINE BINDING-SITES FROM CALF STRIATUM

被引：4

作者：

ROTONDO, A ^{[1
]}

GIANNACCINI, G ^{[1
]}

QUATTRONE, C ^{[1
]}

MARAZZITI, D ^{[1
]}

MARTINI, C ^{[1
]}

CASSANO, GB ^{[1
]}

LUCACCHINI, A ^{[1
]}

机构：

[1] UNIV PISA,IST POLICATTEDRA DISCIPLINE BIOL,I-56100 PISA,ITALY

来源：

NEUROCHEMICAL RESEARCH | 1994年 / 19卷 / 10期

关键词：

SEROTONIN; NEURONAL SEROTONIN TRANSPORTER; CALF STRIATUM; H-3] IMIPRAMINE BINDING; H-3] PAROXETINE BINDING; PURIFICATION;

D O I：

10.1007/BF01006821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The serotonin (5-HT) transporter from calf striatum cerebral membranes was solubilized with digitonin and characterized by gel exclusion chromatography. [H-3]Imipramine and [H-3]paroxetine were utilized as markers for labeling it. H-3-imipramine labels a high- and a low-affinity site on striatum membranes, whereas it binds to a single high-affinity site on the solubilized fraction. [H-3]Paroxetine binds with the same affinity to a single site on both membranes and solubilized preparations. After gel exclusion chromatography of the solubilizate both [H-3]imipramine rind [H-3]paroxetine bind on an identical fraction of 205 kDa molecular weight, with a similar maximum number of binding sites (Bmax). Our results suggest that both H-3-imipramine and [H-3]paroxetine bind to a common site on the 5-HT transporter.

引用

页码：1295 / 1300

页数：6

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