ELEVATED NUMBERS OF PROLIFERATING MONONUCLEAR-CELLS IN THE PERIPHERAL-BLOOD OF PSORIATIC PATIENTS CORRELATE WITH DISEASE SEVERITY

被引:29
作者
JEFFRIES, EWB
LEE, GC
SAID, S
SABAHI, M
MCCULLOUGH, JL
HERROD, R
ALZONA, CP
LINDEN, KG
SOUNDARAJAN, D
EDWARDS, S
WEINSTEIN, GD
机构
[1] VET ADM MED CTR, DEPT DERMATOL, LONG BEACH, CA 90822 USA
[2] UNIV CALIF IRVINE, DEPT DERMATOL, IRVINE, CA 92717 USA
关键词
PROLIFERATION; ACTIVATION; LYMPHOCYTE; METHOTREXATE;
D O I
10.1111/1523-1747.ep12325496
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Although psoriasis is characterized by the accumulation of activated proliferating lymphoid cells in the psoriatic skin lesion, it is not known whether these cells are activated and proliferating before entry into the psoriatic plaque, The current study evaluates the number and phenotype of proliferating lymphoid cells in the blood of psoriatic patients, Proliferation of peripheral blood mononuclear cells was evaluated on cytospun preparations of these cells using autoradiographic techniques after pulsing the mononuclear cells with H-3-methyl thymidine for 2 h. The phenotypes of the labeled peripheral blood mononuclear cells were determined combining autoradiography and immunohistochemistry with monoclonal antibodies directed at CD3, CD4, CD8, CD11c, CD22, and human leukocyte antigen-DR, The data demonstrated elevated numbers of proliferating lymphoid cells in the blood of psoriatic patients compared with normal nonpsoriatic volunteers (p < 0.01). Furthermore, the number of circulating proliferating mononuclear cells increased significantly with increasing psoriasis skin disease severity (correlation coefficient 0.95; p < 0.0001), When the phenotype of the proliferating cells in the blood was examined, the numbers of T cells (CD3(+), CD4(+), CD8(+) cells), B cells (CD22(+) cells), monocytes (CD11c(+) cells), and human leukocyte antigen-DR(+) cells were significantly elevated compared with nonpsoriatic skin (p < 0.01) and increased with increasing disease activity (correlation coefficient range 0.48-0.74; p < 0.05). The data suggest a generalized systemic activation of T, B, and monocytic cells that results in labeling of up to 0.16% of the circulating mononuclear cells with H-3-methyl thymidine (i.e., proliferating and presumably activated) when assayed in vitro.
引用
收藏
页码:733 / 738
页数:6
相关论文
共 35 条
[1]   SPONTANEOUS DNA-SYNTHESIS IN RHEUMATOID-ARTHRITIS - EVIDENCE OF ENHANCED CIRCULATING NON-T-CELL PROLIFERATION [J].
ALBALAGHI, S ;
STROM, H ;
MOLLER, E .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1983, 17 (06) :521-530
[2]   T-CELL SUBPOPULATIONS IN THE BLOOD AND SKIN OF PATIENTS WITH PSORIASIS [J].
BAKER, BS ;
SWAIN, AF ;
VALDIMARSSON, H ;
FRY, L .
BRITISH JOURNAL OF DERMATOLOGY, 1984, 110 (01) :37-44
[3]   PERCUTANEOUS-ABSORPTION OF METHOTREXATE - EFFECT ON EPIDERMAL DNA-SYNTHESIS IN HAIRLESS MICE [J].
BALL, MA ;
MCCULLOUGH, JL ;
WEINSTEIN, GD .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1982, 79 (01) :7-10
[4]  
BJERRING P, 1986, ACTA DERM-VENEREOL, V66, P515
[5]  
Borroni G, 1989, Acta Derm Venereol Suppl (Stockh), V146, P125
[6]  
BRETT SJ, 1987, CLIN EXP IMMUNOL, V68, P510
[7]   INTRALESIONAL CYCLOSPORINE FOR PSORIASIS - RELATIONSHIP OF DOSE, TISSUE-LEVELS, AND EFFICACY [J].
BURNS, MK ;
ELLIS, CN ;
EISEN, D ;
DUELL, E ;
GRIFFITHS, CEM ;
ANNESELEY, TM ;
HAMILTON, TA ;
BIRNBAUM, JE ;
VOORHEES, JJ .
ARCHIVES OF DERMATOLOGY, 1992, 128 (06) :786-790
[8]   RESPONSE OF IMMUNOREGULATORY LYMPHOCYTE SUBSETS TO METHOTREXATE IN RHEUMATOID-ARTHRITIS [J].
CALABRESE, LH ;
TAYLOR, JV ;
WILKE, WS ;
SEGAL, AM ;
VALENZUELA, R ;
CLOUGH, JD .
CLEVELAND CLINIC JOURNAL OF MEDICINE, 1990, 57 (03) :232-241
[9]   CD8+ T-CELLS IN PSORIATIC LESIONS PREFERENTIALLY USE T-CELL RECEPTOR V(BETA)3 AND/OR V(BETA)13.1 GENES [J].
CHANG, JCC ;
SMITH, LR ;
FRONING, KJ ;
SCHWABE, BJ ;
LAXER, JA ;
CARALLI, LL ;
KURLAND, HH ;
KARASEK, MA ;
WILKINSON, DI ;
CARLO, DJ ;
BROSTOFF, SW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9282-9286
[10]   PRECURSOR FREQUENCY OF ANTIGEN-SPECIFIC T-CELLS - EFFECTS OF SENSITIZATION INVIVO AND INVITRO [J].
FORD, D ;
BURGER, D .
CELLULAR IMMUNOLOGY, 1983, 79 (02) :334-344