CONSTRUCTION OF A YEAST ARTIFICIAL CHROMOSOME CONTIG SPANNING THE PSEUDOAUTOSOMAL REGION AND ISOLATION OF 25 NEW SEQUENCE-TAGGED SITES

被引：26

作者：

SLIM, R

LEPASLIER, D

COMPAIN, S

LEVILLIERS, J

OUGEN, P

BILLAULT, A

DONOHUE, SJ

KLEIN, DC

MINTZ, L

BERNHEIM, A

COHEN, D

WEISSENBACH, J

PETIT, C

机构：

[1] INST PASTEUR,UNITE GENET MOLEC HUMAINE,CNRS,URA 1445,25 RUE DR ROUX,F-75724 PARIS 15,FRANCE

[2] CTR ETUD POLYMORPHISME HUMAIN,F-75010 PARIS,FRANCE

[3] NICHHD,NEUROENDOCRINOL SECT,DEV NEUROBIOL LAB,BETHESDA,MD 20892

[4] INST GUSTAVE ROUSSY,CYTOGENET & GENET ONCOL LAB,CNRS,URA 1158,F-94805 VILLEJUIF,FRANCE

来源：

GENOMICS | 1993年 / 16卷 / 03期

关键词：

D O I：

10.1006/geno.1993.1249

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thirty-one yeast artificial chromosomes (YACs) from the human pseudoautosomal region were identified by a combination of sequence-tagged site (STS) screenings and colony hybridizations, using a subtelomeric interspersed repetitive element mapping predominantly to the pseudoautosomal region. Twenty-five new pseudoautosomal STSs were generated, of which 4 detected restriction fragment length polymorphisms. A total of 33 STSs were used to assemble the 31 YACs into a single contiguous set of overlapping DNA fragments spanning at least 2.3 megabases of the pseudoautosomal region. In addition, four pseudoautosomal genes including hydroxyindole O-methyltransferase have been positioned on this set of fragments. © 1993 Academic Press. All rights reserved.

引用

收藏

页码：691 / 697

页数：7

相关论文

共 48 条

[1] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].

ALBERTSEN, HM ;

ABDERRAHIM, H ;

CANN, HM ;

DAUSSET, J ;

LEPASLIER, D ;

COHEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260

[2] CONTIGUOUS GENE SYNDROMES DUE TO DELETIONS IN THE DISTAL SHORT ARM OF THE HUMAN X-CHROMOSOME [J].

BALLABIO, A ;

BARDONI, B ;

CARROZZO, R ;

ANDRIA, G ;

BICK, D ;

CAMPBELL, L ;

HAMEL, B ;

FERGUSONSMITH, MA ;

GIMELLI, G ;

FRACCARO, M ;

MARASCHIO, P ;

ZUFFARDI, O ;

GUIOLI, S ;

CAMERINO, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :10001-10005

[3] CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME CONTIG SPANNING THE HUNTINGTONS-DISEASE GENE CANDIDATE REGION [J].

BATES, GP ;

VALDES, J ;

HUMMERICH, H ;

BAXENDALE, S ;

LEPASLIER, DL ;

MONACO, AP ;

TAGLE, D ;

MACDONALD, ME ;

ALTHERR, M ;

ROSS, M ;

BROWNSTEIN, BH ;

BENTLEY, D ;

WASMUTH, JJ ;

GUSELLA, JF ;

COHEN, D ;

COLLINS, F ;

LEHRACH, H .

NATURE GENETICS, 1992, 1 (03) :180-187

[4] A PHYSICAL MAP OF THE HUMAN PSEUDOAUTOSOMAL REGION [J].

BROWN, WRA .

EMBO JOURNAL, 1988, 7 (08) :2377-2385

[5] ISOLATION OF SINGLE-COPY HUMAN GENES FROM A LIBRARY OF YEAST ARTIFICIAL CHROMOSOME CLONES [J].

BROWNSTEIN, BH ;

SILVERMAN, GA ;

LITTLE, RD ;

BURKE, DT ;

KORSMEYER, SJ ;

SCHLESSINGER, D ;

OLSON, MV .

SCIENCE, 1989, 244 (4910) :1348-1351

[6] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[7] HYPERVARIABLE TELOMERIC SEQUENCES FROM THE HUMAN SEX-CHROMOSOMES ARE PSEUDOAUTOSOMAL [J].

COOKE, HJ ;

BROWN, WRA ;

RAPPOLD, GA .

NATURE, 1985, 317 (6039) :687-692

[8] DNA-SEQUENCES OF 2 EXPRESSED NUCLEAR GENES FOR HUMAN MITOCHONDRIAL ADP ATP TRANSLOCASE [J].

COZENS, AL ;

RUNSWICK, MJ ;

WALKER, JE .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 206 (02) :261-280

[9] EVIDENCE FOR A PSEUDOAUTOSOMAL LOCUS FOR SCHIZOPHRENIA .2. REPLICATION OF A NONRANDOM SEGREGATION OF ALLELES AT THE DXYS14 LOCUS [J].

DAMATO, T ;

CAMPION, D ;

GORWOOD, P ;

JAY, M ;

SABATE, O ;

PETIT, C ;

ABBAR, M ;

MALAFOSSE, A ;

LEBOYER, M ;

HILLAIRE, D ;

CLERGETDARPOUX, F ;

FEINGOLD, J ;

WAKSMAN, G ;

MALLET, J .

BRITISH JOURNAL OF PSYCHIATRY, 1992, 161 :59-62

[10] REPORT OF THE COMMITTEE-ON-THE-GENETIC-CONSTITUTION-OF-THE-X-CHROMOSOME [J].

DAVIES, KE ;

MANDEL, JL ;

MONACO, AP ;

NUSSBAUM, RL ;

WILLARD, HF .

CYTOGENETICS AND CELL GENETICS, 1991, 58 (1-2) :853-966

← 1 2 3 4 5 →