COLLAGENASE DIGESTION OF BONE-MARROW TREPHINE BIOPSY SPECIMENS - AN IMPORTANT ADJUNCT TO HEMATOLOGICAL DIAGNOSIS WHEN MARROW ASPIRATION FAILS

被引：5

作者：

MAUNG, ZT ^{[1
]}

BOWN, NP ^{[1
]}

HAMILTON, PJ ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,DIV HUMAN GENET,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1993年 / 46卷 / 06期

关键词：

D O I：

10.1136/jcp.46.6.576

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Failure to obtain sufficient material from marrow aspiration (dry tap) posed a diagnostic problem in two patients with pancytopenia. By using collagenase digestion of the trephine biopsy specimen, a precise diagnosis was reached. This technique is very useful because it permits flow cytometric and immunocytochemical analyses of cell suspensions obtained after collagenase digestion of the trephine biopsy specimen core. Acute leukaemia presenting with a dry tap can therefore be accurately immunophenotyped. The technique is easy to perform and merits wider use.

引用

页码：576 / 577

页数：2

共 10 条

[1] BENNETT JM, 1982, BRIT J HAEMATOL, V51, P185
[2] VITAMIN-B12 AND FOLATE-DEFICIENCY PRESENTING AS LEUKEMIA
DOKAL, IS
COX, TM
GALTON, DAG
[J]. BRITISH MEDICAL JOURNAL, 1990, 300 (6734) : 1263 - 1264
[3] COLLAGENASE DIGESTION OF TREPHINE BIOPSIES - RAPID DIAGNOSIS OF THE DRY TAP WITH PRESERVATION OF CYTOCHEMICAL REACTIVITY
GIBSON, J
GRIMMETT, K
JOSHUA, DE
KRONENBERG, H
[J]. PATHOLOGY, 1988, 20 (02) : 200 - 201
[4] DRY TAP BONE-MARROW ASPIRATION - CLINICAL-SIGNIFICANCE
HUMPHRIES, JE
[J]. AMERICAN JOURNAL OF HEMATOLOGY, 1990, 35 (04) : 247 - 250
[5] MYELODYSPLASTIC SYNDROME WITH INCREASED MARROW FIBROSIS - A DISTINCT CLINICOPATHOLOGICAL ENTITY
LAMBERTENGHIDELILIERS, G
ORAZI, A
LUKSCH, R
ANNALORO, C
SOLIGO, D
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1991, 78 (02) : 161 - 166
[6] VITAMIN-B12 AND FOLATE-DEFICIENCY PRESENTING AS LEUKEMIA
LENNARD, A
[J]. BRITISH MEDICAL JOURNAL, 1990, 301 (6744) : 180 - 180
[7] USE OF BONE CORE BIOPSIES FOR CYTOGENETIC ANALYSIS
MARTIN, P
ROWLEY, JD
BARON, JM
[J]. HUMAN GENETICS, 1979, 51 (02) : 163 - 166
[8] A RAPID PROCEDURE, BY COLLAGENASE TREATMENT AND CYTOCENTRIFUGATION, FOR THE CYTOLOGICAL EVALUATION OF BONE-MARROW BIOPSY SPECIMENS
MONONEN, I
JANSSON, SE
[J]. CLINICAL AND LABORATORY HAEMATOLOGY, 1986, 8 (02): : 149 - 154
[9] BONE-MARROW HISTOLOGY IN MYELODYSPLASTIC SYNDROMES .1. HISTOLOGICAL-FINDINGS IN MYELODYSPLASTIC SYNDROMES AND COMPARISON WITH BONE-MARROW SMEARS
TRICOT, G
DEWOLFPEETERS, C
HENDRICKX, B
VERWILGHEN, RL
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1984, 57 (03) : 423 - 430
[10] BONE-MARROW HISTOLOGY-3 - VALUE OF BONE-MARROW CORE BIOPSY IN ACUTE-LEUKEMIA, MYELODYSPLASTIC SYNDROMES, AND CHRONIC MYELOID-LEUKEMIA
WINFIELD, DA
POLACARZ, SV
[J]. JOURNAL OF CLINICAL PATHOLOGY, 1992, 45 (10) : 855 - 859

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