DETERMINATION OF TRACES OF NATURAL BRASSINOSTEROIDS AS DANSYLAMINOPHENYLBORONATES BY LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION

被引：40
作者：
GAMOH, K
OKAMOTO, N
TAKATSUTO, S
TEJIMA, I
机构：
[1] JOETSU UNIV EDUC,DEPT CHEM,JOETSU 943,JAPAN
[2] NIPPON KAYAKU CO LTD,AGEO RES LAB,AGEO 362,JAPAN
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 228卷 / 01期
关键词：
D O I：
10.1016/S0003-2670(00)80484-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A microanalytical method for the determination of traces of natural brassinosteroids as their dansylaminophenylboronates by liquid chromatography with fluorimetric detection is described. The boronates are easily prepared by the reaction of brassinosteroids with dansylaminophenylboronic acid (DABA), which was newly synthesized as a precolumn fluorogenic reagent for brassinosteroids. The potential of the method is demonstrated by the separation of a standard brassinosteroid mixture and the analysis of a biologically active fraction sample obtained from an extract of Zea mays L. pollen. The detection limit of the DABA derivative was found to be 25 pg for brassinolide, which is superior to that of the phenanthreneboronate derivative. © 1990.
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页码：101 / 105
页数：5
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