DETECTION OF RABIES VIRUS MESSENGER-RNA IN MOUSE-BRAIN BY USING INSITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED RNA PROBES

被引：12
|
作者：
JACKSON, AC [1 ]
机构：
[1] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3J7, ONTARIO, CANADA
来源：
MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 02期
基金：
英国医学研究理事会;
关键词：
ENCEPHALITIS; INSITU HYBRIDIZATION; NONISOTOPIC; NUCLEIC ACID HYBRIDIZATION; RABIES;
D O I：
10.1016/0890-8508(92)90057-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A non-isotopic method of in situ hybridization (ISH) was developed for the detection of rabies virus RNA in paraffin-embedded tissues. Digoxigenin-labelled RNA probes for rabies virus glycoprotein mRNA were used. The method had good sensitivity and low backgrounds, and there was excellent cellular localization of signals. ISH wih digoxigenin-labelled probes was compared with ISH with 3H-labelled probes. This non-isotopic method of ISH is more convenient than the radiolabelled method, and it is quicker because a long autoradiographic exposure is not required. © 1992.
引用
收藏
页码：131 / 136
页数：6
相关论文
共 50 条
  • [41] INSITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED PROBES - SENSITIVE AND RELIABLE DETECTION METHOD APPLIED TO MYELINATING RAT-BRAIN
    BREITSCHOPF, H
    SUCHANEK, G
    GOULD, RM
    COLMAN, DR
    LASSMANN, H
    ACTA NEUROPATHOLOGICA, 1992, 84 (06) : 581 - 587
  • [42] DETECTION OF CYTOKINE MESSENGER-RNA IN RETINOBLASTOMA USING INSITU HYBRIDIZATION
    LIN, RT
    KALETAMICHAELS, S
    CHAR, BH
    CLINICAL RESEARCH, 1992, 40 (01): : A62 - A62
  • [43] SENSITIVE NORTHERN BLOT HYBRIDIZATION USING DIGOXIGENIN RNA PROBES FOR THE MESSENGER-RNA DETECTION OF 2 GLUCOSE-TRANSPORTER ISOFORMS
    NIVET, V
    HAJDUCH, E
    HAINAULT, I
    DELATTRE, J
    LAVAU, M
    HAINQUE, B
    CELLULAR AND MOLECULAR BIOLOGY, 1995, 41 (07) : 979 - 984
  • [44] Nonradioactive Northern blotting with biotinylated and digoxigenin-labeled RNA probes
    Meltzer, JC
    Sanders, V
    Grimm, PC
    Chiasson, N
    Hoeltke, HJ
    Garrett, KL
    Greenberg, AH
    Nance, DM
    ELECTROPHORESIS, 1998, 19 (8-9) : 1351 - 1355
  • [45] DETECTION OF PROOPIOMELANOCORTIN MESSENGER-RNA USING A DIGOXIGENIN END LABELED OLIGODEOXYNUCLEOTIDE PROBE
    FARQUHARSON, M
    HARVIE, R
    MCNICOL, AM
    JOURNAL OF PATHOLOGY, 1990, 160 (02): : A168 - A168
  • [46] VISUALIZATION OF PREPROCHOLECYSTOKININ MESSENGER-RNA IN RAT-BRAIN BY INSITU HYBRIDIZATION USING COMPLEMENTARY-DNA AND RNA PROBES
    DEBELLEROCHE, J
    OBRIEN, K
    PREMI, BK
    MALCOLM, ADB
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 177 - 177
  • [47] DETECTION OF NEUROPEPTIDE MESSENGER-RNA IN RAT-BRAIN BY INSITU HYBRIDIZATION WITH RADIOLABELED SYNTHETIC OLIGONUCLEOTIDE PROBES
    ARENTZEN, R
    MANNING, RW
    WOLFSON, B
    DAVIS, LG
    HIGGINS, GA
    LIN, Y
    BALDINO, F
    NUCLEOSIDES & NUCLEOTIDES, 1985, 4 (1-2): : 261 - 261
  • [48] DETECTION OF HUMAN PAPILLOMA-VIRUS (HPV) DNA IN GENITAL BIOPSY SPECIMENS BY INSITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED PROBES
    HEINO, P
    HUKKANEN, V
    ARSTILA, P
    JOURNAL OF VIROLOGICAL METHODS, 1989, 26 (03) : 331 - 337
  • [49] COMPARISON OF S-35 AND DIGOXIGENIN-LABELED RNA AND OLIGONUCLEOTIDE PROBES FOR INSITU HYBRIDIZATION - EXPRESSION OF MESSENGER-RNA OF THE SEMINAL-VESICLE SECRETION PROTEIN-II AND ANDROGEN RECEPTOR GENES IN THE RAT PROSTATE
    KOMMINOTH, P
    MERK, FB
    LEAV, I
    WOLFE, HJ
    ROTH, J
    HISTOCHEMISTRY, 1992, 98 (04) : 217 - 228
  • [50] INSITU HYBRIDIZATION OF ALBUMIN MESSENGER-RNA IN NORMAL LIVER AND HEPATOCELLULAR-CARCINOMA WITH A DIGOXIGENIN LABELED OLIGONUCLEOTIDE PROBE
    MURRAY, GI
    PATERSON, PJ
    EWEN, SWB
    MELVIN, WT
    JOURNAL OF CLINICAL PATHOLOGY, 1992, 45 (01) : 21 - 24
12345