DETECTION OF RABIES VIRUS MESSENGER-RNA IN MOUSE-BRAIN BY USING INSITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED RNA PROBES

被引：12
|
作者：
JACKSON, AC [1 ]
机构：
[1] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3J7, ONTARIO, CANADA
来源：
MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 02期
基金：
英国医学研究理事会;
关键词：
ENCEPHALITIS; INSITU HYBRIDIZATION; NONISOTOPIC; NUCLEIC ACID HYBRIDIZATION; RABIES;
D O I：
10.1016/0890-8508(92)90057-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A non-isotopic method of in situ hybridization (ISH) was developed for the detection of rabies virus RNA in paraffin-embedded tissues. Digoxigenin-labelled RNA probes for rabies virus glycoprotein mRNA were used. The method had good sensitivity and low backgrounds, and there was excellent cellular localization of signals. ISH wih digoxigenin-labelled probes was compared with ISH with 3H-labelled probes. This non-isotopic method of ISH is more convenient than the radiolabelled method, and it is quicker because a long autoradiographic exposure is not required. © 1992.
引用
收藏
页码：131 / 136
页数：6
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