DETECTION OF RABIES VIRUS MESSENGER-RNA IN MOUSE-BRAIN BY USING INSITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED RNA PROBES

被引：12
作者：
JACKSON, AC [1 ]
机构：
[1] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3J7, ONTARIO, CANADA
来源：
MOLECULAR AND CELLULAR PROBES | 1992年 / 6卷 / 02期
基金：
英国医学研究理事会;
关键词：
ENCEPHALITIS; INSITU HYBRIDIZATION; NONISOTOPIC; NUCLEIC ACID HYBRIDIZATION; RABIES;
D O I：
10.1016/0890-8508(92)90057-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A non-isotopic method of in situ hybridization (ISH) was developed for the detection of rabies virus RNA in paraffin-embedded tissues. Digoxigenin-labelled RNA probes for rabies virus glycoprotein mRNA were used. The method had good sensitivity and low backgrounds, and there was excellent cellular localization of signals. ISH wih digoxigenin-labelled probes was compared with ISH with 3H-labelled probes. This non-isotopic method of ISH is more convenient than the radiolabelled method, and it is quicker because a long autoradiographic exposure is not required. © 1992.
引用
收藏
页码：131 / 136
页数：6
相关论文
共 28 条
  • [1] INSITU HYBRIDIZATION - AN OPTIMIZED DETECTION PROTOCOL FOR A BIOTINYLATED DNA PROBE RENDERS IT MORE SENSITIVE THAN A COMPARABLE S-35-LABELED PROBE
    ALLAN, GM
    TODD, D
    SMYTH, JA
    MACKIE, DP
    BURNS, J
    MCNULTY, MS
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1989, 24 (1-2) : 181 - 190
  • [2] ANGERER LM, 1987, IN SITU HYBRIDIZATIO, P42
  • [3] [Anonymous], CARLETONS HIST TECHN
  • [4] BALDINO F, 1989, METHODS NEUROSCIENCE, V1, P282
  • [5] Bochenek B, 1990, PLANT MOL BIOL REP, V8, P237
  • [6] Dean D. J., 1973, Laboratory techniques in rabies. WHO Monograph Series No.23., P73
  • [7] FEIDEN W, 1985, CLIN NEUROPATHOL, V4, P156
  • [8] IMMUNOHISTOCHEMICAL STAINING OF RABIES VIRUS-ANTIGEN WITH MONOCLONAL AND POLYCLONAL ANTIBODIES IN PARAFFIN TISSUE-SECTIONS
    FEIDEN, W
    KAISER, E
    GERHARD, L
    DAHME, E
    GYLSTORFF, B
    WANDELER, A
    EHRENSBERGER, F
    [J]. JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1988, 35 (04): : 247 - 255
  • [9] USE OF THE AVIDIN BIOTIN PEROXIDASE SYSTEM TO DETECT RABIES ANTIGEN IN FORMALIN-FIXED PARAFFIN-EMBEDDED TISSUES
    FEKADU, M
    GREER, PW
    CHANDLER, FW
    SANDERLIN, DW
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1988, 19 (02) : 91 - 96
  • [10] HEMMATIBRIVANLOU A, 1990, DEVELOPMENT, V110, P325
123