A SIMPLE ASSAY FOR PROTEOLYSIS OF IGFBP-3

被引：173
作者：
LAMSON, G
GIUDICE, LC
ROSENFELD, RG
机构：
[1] STANFORD UNIV, MED CTR, DEPT PEDIAT, DIV ENDOCRINOL, STANFORD, CA 94305 USA
[2] STANFORD UNIV, MED CTR, DEPT OBSTET & GYNECOL, DIV REPROD ENDOCRINOL, STANFORD, CA 94305 USA
来源：
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1991年 / 72卷 / 06期
关键词：
D O I：
10.1210/jcem-72-6-1391
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using I-125 hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37-degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
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页码：1391 / 1393
页数：3
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