CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS

被引：14

作者：

RUITERS, MHJ

VANSPRONSEN, EA

SKJELDAL, OH

STROMME, P

SCHOLTE, HR

PZYREMBEL, H

SMIT, GPA

RUITENBEEK, W

AGSTERIBBE, E

机构：

[1] UNIV GRONINGEN,PHYSIOL CHEM LAB,BLOEMSINGEL 10,9712 KZ GRONINGEN,NETHERLANDS

[2] UNIV AMSTERDAM,DEPT MOLEC CELL BIOL,MOLEC CYTOL SECT,AMSTERDAM,NETHERLANDS

[3] UNIV OSLO,RIKSHOSP,CTR MENTAL RETARDAT,DEPT PAEDIAT,OSLO,NORWAY

[4] ERASMUS UNIV,DEPT BIOCHEM,3000 DR ROTTERDAM,NETHERLANDS

[5] ERASMUS UNIV,SOPHIAS CHILDRENS HOSP,DEPT PAEDIAT,3000 DR ROTTERDAM,NETHERLANDS

[6] STATE UNIV GRONINGEN,DEPT PAEDIAT,9700 AB GRONINGEN,NETHERLANDS

[7] CATHOLIC UNIV NIJMEGEN,DEPT PAEDIAT,NIJMEGEN,NETHERLANDS

来源：

JOURNAL OF INHERITED METABOLIC DISEASE | 1991年 / 14卷 / 01期

关键词：

D O I：

10.1007/BF01804387

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethyl-aminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

引用

页码：45 / 48

页数：4

共 5 条

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WIJBURG FA, 1989, BIOCHEM INT, V19, P563

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