Inhibition by Methylprednisolone Acetate Suggests an Indirect Mechanism for TGF-B Induced Angiogenesis

被引:23
|
作者
Phillips, Gregg D. [1 ]
Whitehead, Russell A. [2 ]
Knighton, David R. [1 ]
机构
[1] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
[2] Macalester Coll, Dept Biol, St Paul, MN 55105 USA
关键词
D O I
10.3109/08977199209008873
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Angiogenesis induced by transforming growth factor beta (TGFB) implanted in the rabbit cornea is accompanied by an influx of inflammatory cells. To determine if the inflammatory cells are the mediators of the neovascularization, they were depleted by local administration of methylprednisolone acetate (MPA). Subconjunctival injections of 16 mg of MPA immediately following implantation of 50 ng of TGFB in the cornea prevented the inflammation and subsequent formation of capillaries. If the injections of MPA were delayed by 48 hr and the inflammatory cells were allowed to enter the cornea, angiogenesis occured, demonstrating that MPA had no adverse effects on the ability of endothelial cells to form capillaries. These results confirm the hypothesis that TGFB induces angiogenesis indirectly by recruiting inflammatory cells capable of stimulating direct angiogenesis.
引用
收藏
页码:77 / 84
页数:9
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