Extraction of Genomic DNA Using Magnetic Nanoparticles (Fe3O4) as a Solid-Phase Support

被引：10
作者：
Saiyed, Z. M. [1 ]
Ramchand, C. N. [2 ]
机构：
[1] Univ Baroda, Dept Biochem, Vadodara, India
[2] Kemim Nutr Technol India Pvt Ltd, 39 Trapezium,Nelson Manickam Rd, Chennai 600029, Tamil Nadu, India
来源：
AMERICAN JOURNAL OF INFECTIOUS DISEASES | 2007年 / 3卷 / 04期
关键词：
magnetic nanoparticles; mammalian cells; PCR; agarose gel;
D O I：
10.3844/ajidsp.2007.225.229
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Magnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to the conventional procedures in all the samples tested. Additionally, the magnetic method takes less than 15 minutes to extract DNA as against several hours taken by conventional protocols. Furthermore, the isolated DNA was found to function satisfactorily in PCR amplification and restriction endonuclease digestion. The developed procedure is simple, quick, cheap, robust and does not require the use of organic solvents or sophisticated equipments; thereby making it more amenable to automation.
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收藏
页码：225 / 229
页数：5
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