CLONING AND FUNCTIONAL EXPRESSION OF A CARDIAC INWARD RECTIFIER K+ CHANNEL

被引：39

作者：

ISHII, K

YAMAGISHI, T

TAIRA, N

机构：

[1] Department of Pharmacology, Tohoku University School of Medicine, Aoba-ku, Sendai, 980

来源：

FEBS LETTERS | 1994年 / 338卷 / 01期

关键词：

K+ CHANNEL; INWARD RECTIFIER; HEART; CDNA CLONING; RNA BLOT ANALYSIS;

D O I：

10.1016/0014-5793(94)80126-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA coding for an inward rectifier K+ channel (RBHIK1) from rabbit heart. The cloned cDNA encodes a protein of 427 amino acids with two putative transmembrane segments. The primary structure of RBHIK1 is highly homologous to that of IRK1 which is an inward rectifier K+ channel recently cloned from mouse macrophage by expression cloning. When expressed in Xenopus oocytes, RBHIK1 current showed strong inward rectification and was inhibited by extracellular Ba2+ and Cs+. RNA blot analysis revealed the expression of RBHIK1 mRNA in various rabbit tissues, especially high level in the ventricular muscle.

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页码：107 / 111

页数：5

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