The ultrastructure of basal lamina (BL) of retinal pigment epithelium (RPE) in guinea gigs was studied by the quick-freezing, deep-etching (QF-DE) method. The retinal tissue was quickly frozen, fractured and deeply etched. A replica was prepared by shadowing with platinum and carbon. Electron micrographs of the BL of RPE provide a three-dimensional (3-D) ultrastructure. The lamina lucida was filled with traversing filamentous structures, which connected the basal infolding of RPE with the lamina densa. The lamina densa consisted of a 3-D meshwork structure. It is suggested that the traversing filamentous structures in the lamina lucida might play a role in anchoring the basal infolding of RPE and in maintaining the normal architecture.
Ping Zhang Guanguang Feng Tao Yue Jianxian Lin Yuzhen Yi Youjian PangZhongshan Ophthalmic Center Biochemistry DepartmentSun Yatsen University of Medical Sciences Guangzhou China
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Ping Zhang Guanguang Feng Tao Yue Jianxian Lin Yuzhen Yi Youjian PangZhongshan Ophthalmic Center Biochemistry DepartmentSun Yatsen University of Medical Sciences Guangzhou China
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SWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDENSWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDEN
WRIGSTAD, A
NILSSON, SEG
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SWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDENSWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDEN
NILSSON, SEG
NARFSTROM, K
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SWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDENSWEDISH UNIV AGR SCI, FAC VET MED, DEPT SURG & MED, S-75007 UPPSALA, SWEDEN