PRESSURE-DROP CORRELATIONS AND SCALE-UP OF SIZE EXCLUSION CHROMATOGRAPHY WITH COMPRESSIBLE PACKINGS

Pressure drop measurements were made for compressible gel packings Sephadex G-25 and G-100. A critical velocity at which pressure drop kept increasing was observed. Correlations were developed for pressure drop. Measurements of the chromatographic separation obtained for separating bovine serum albumin and nickel nitrate were correlated as measurements of H. A scaling procedure which keeps separation, pressure drop, and feed throughput constant was developed for each packing. The procedure and results for G-25 are similar to those developed previously for rigid packings. The use of smaller diameter packings can greatly reduce the amount of packing required. For G-100, which is quite compressible, the scaling procedure was different. Results of the scaling analysis for G-100 showed that there is little advantage to decreasing the particle size with very compressible packings.