A METHOD FOR PRODUCTION OF ANTIBODIES TO HUMAN T-CELL RECEPTOR BETA-CHAIN VARIABLE REGIONS

被引：38
作者：
CHOI, YW
KOTZIN, B
LAFFERTY, J
WHITE, J
PIGEON, M
KUBO, R
KAPPLER, J
MARRACK, P
机构：
[1] UNIV COLORADO,HLTH SCI CTR,NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT,DENVER,CO 80206
[2] UNIV COLORADO,HLTH SCI CTR,DEPT BIOCHEM BIOPHYS & GENET,DENVER,CO 80206
[3] UNIV COLORADO,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL,DENVER,CO 80206
[4] UNIV COLORADO,HLTH SCI CTR,DEPT MED,DENVER,CO 80206
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 19期
关键词：
D O I：
10.1073/pnas.88.19.8357
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Mouse T-cell hybridomas bearing human V-beta elements were produced by transfection of human/mouse hybrid T-cell receptor beta-chain genes into a mouse T-cell hybridoma lacking an endogenous beta-chain gene. These hybridomas were entirely mouse in origin except for the human V-beta region. These cells were used to immunize mice against human V-beta elements. Mouse monoclonal antibodies have thus been generated against human V-beta-13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V-beta or V-alpha elements.
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页码：8357 / 8361
页数：5
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