MARKED INCREASES IN DT DIAPHORASE ACTIVITY IN MALIGNANTLY TRANSFORMED SYRIAN-HAMSTER FIBROBLASTIC CELLS

被引：5

作者：

TSUDA, H

机构：

[1] Life Science Research Laboratory, Japan Tobacco Inc. 6-2 Umegaoka, Midori-ku, Yokohama, Kanagawa

来源：

CANCER LETTERS | 1991年 / 56卷 / 03期

关键词：

DT DIAPHORASE; SYRIAN HAMSTER CELLS; MALIGNANT CELLS; TUMOR MARKER;

D O I：

10.1016/0304-3835(91)90002-Y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

NAD(P)H quinone oxidoreductase (DT diaphorase; EC 1.6.99.2) activity in cultured Syrian hamster fibroblastic cells was measured. The cells examined were classified into three categories: (1) four primary embryonic fibroblasts, (2) three non-malignant immortal cells and (3) four malignantly transformed cells. The results showed that cytosolic DT diaphorase activity in malignant cells was markedly higher than the corresponding activity in non-malignant cells (enzyme activity: (3) >> (1) greater-than-or-equal-to (2)). The enzyme activity was not influenced very much by the particular phase of cell growth. Thus, the increase in DT diaphorase activity seems to be a good marker for malignant transformation, at least in Syrian hamster fibroblastic cells.

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页码：189 / 195

页数：7

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