ELECTROOXIDATION OF NADH WITH CO(PHEN)3(3+) BY NADH DEHYDROGENASE PURIFIED FROM BACILLUS-STEAROTHERMOPHILUS

The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electro-chemical oxidation of NADH mediated by Co(phen)3(3+). Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3(3+) is 5 x 10(6) M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 x 10(7) M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.