IMMUNOASSAYS FOR THE DETECTION OF HUMAN COLLAGENASE, STROMELYSIN, TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP) AND ENZYME-INHIBITOR COMPLEXES

被引：89

作者：

COOKSLEY, S ^{[1
]}

HIPKISS, JB ^{[1
]}

TICKLE, SP ^{[1
]}

HOLMESIEVERS, E ^{[1
]}

DOCHERTY, AJP ^{[1
]}

MURPHY, G ^{[1
]}

LAWSON, ADG ^{[1
]}

机构：

[1] STRANGEWAYS RES LAB,DEPT CELL PHYSIOL,CAMBRIDGE CB1 4RN,ENGLAND

来源：

MATRIX | 1990年 / 10卷 / 05期

基金：

英国医学研究理事会;

关键词：

collagenase; ELISA; enzyme-inhibitor complexes; metalloproteinase; monoclonal antibody; stromelysin; TIMP;

D O I：

10.1016/S0934-8832(11)80183-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoassays have been developed for human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and TIMP complexed with both of the active enzymes. Selection of antibodies of defined specificity enabled measurement of both the pro and active forms of the metalloproteinase. Free TIMP was quantified by the selection of a monoclonal antibody which did not recognise TIMP when complexed with metalloproteinases. Detection of enzyme-inhibitor complexes was achieved by capturing the TIMP component of the complex and revealing the metalloenzyme using specific antibodies. © 1990, Gustav Fischer Verlag · Stuttgart · New York. All rights reserved.

引用

页码：285 / 291

页数：7

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