A SIMPLE NEW METHOD FOR USING ANTIGENS SEPARATED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS TO STIMULATE LYMPHOCYTES INVITRO AFTER CONVERTING BANDS CUT FROM WESTERN BLOTS INTO ANTIGEN-BEARING PARTICLES

被引:247
作者
ABOUZEID, C
FILLEY, E
STEELE, J
ROOK, GAW
机构
关键词
D O I
10.1016/0022-1759(87)90429-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:5 / 10
页数:6
相关论文
共 7 条
[1]  
ABOUZEID C, 1986, J GEN MICROBIOL, V132, P3047
[2]   INDIA INK STAINING OF PROTEINS ON NITROCELLULOSE PAPER [J].
HANCOCK, K ;
TSANG, VCW .
ANALYTICAL BIOCHEMISTRY, 1983, 133 (01) :157-162
[3]   ELECTROBLOTTING OF MULTIPLE GELS - A SIMPLE APPARATUS WITHOUT BUFFER TANK FOR RAPID TRANSFER OF PROTEINS FROM POLYACRYLAMIDE TO NITROCELLULOSE [J].
KYHSEANDERSEN, J .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1984, 10 (3-4) :203-209
[4]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[5]  
ROOK GAW, 1986, IMMUNOLOGY, V57, P159
[6]  
YOUNG DB, 1986, IMMUNOLOGY, V59, P167
[7]   GENES FOR THE MAJOR PROTEIN ANTIGENS OF THE LEPROSY PARASITE MYCOBACTERIUM-LEPRAE [J].
YOUNG, RA ;
MEHRA, V ;
SWEETSER, D ;
BUCHANAN, T ;
CLARKCURTISS, J ;
DAVIS, RW ;
BLOOM, BR .
NATURE, 1985, 316 (6027) :450-452