BIOCHEMICAL ASPECTS OF CAROTENE DESATURATION AND CYCLIZATION IN CHROMOPLAST MEMBRANES FROM NARCISSUS-PSEUDONARCISSUS

被引：19

作者：

BEYER, P

NIEVELSTEIN, V

ALBABILI, S

BONK, M

KLEINIG, H

机构：

[1] Institut fur Biologie II, Universität Freiburg, Schanzlestr. 1

来源：

PURE AND APPLIED CHEMISTRY | 1994年 / 66卷 / 05期

关键词：

D O I：

10.1351/pac199466051047

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In Narcissus chromoplast membranes, phytoene is converted into coloured carotenes by two desaturases which differ in stereospecificity and which can be distinguished by herbicidal compounds. The desaturases represent flavoproteins, which gain FAD in an assisted folding pathway after being imported into the organelle. Electrons liberated in the desaturation are transferred to oxygen via an intermediate quinone carrier. The possibility of a co-reduction mechanism is discussed. The redox-state of quinones is regulated by an NADPH: quinone oxidoreductase which is membrane-peripherally localized. The end product of the desaturation, 7,9,7',9,-tetra-cis-lycopene (prolycopene), is the preferred substrate for cyclization, which is accompanied by an NADPH-dependent isomerization yielding trans cyclic products.

引用

页码：1047 / 1056

页数：10

共 15 条

[1] MOLECULAR-CLONING AND EXPRESSION IN PHOTOSYNTHETIC BACTERIA OF A SOYBEAN CDNA CODING FOR PHYTOENE DESATURASE, AN ENZYME OF THE CAROTENOID BIOSYNTHESIS PATHWAY [J].