INDUCTION OF APOPTOTIC DNA FRAGMENTATION AND CELL-DEATH BY NATURAL CERAMIDE

被引：159

作者：

JI, L

ZHANG, G

UEMATSU, S

AKAHORI, Y

HIRABAYASHI, Y

机构：

[1] INST PHYS & CHEM RES,FRONTIER RES PROGRAM,GLYCO CELL BIOL LAB,WAKO,SAITAMA 35101,JAPAN

[2] UNIV SHIZUOKA,SCH PHARMACEUT SCI,PHYS CHEM LAB,SHIZUOKA 422,JAPAN

来源：

FEBS LETTERS | 1995年 / 358卷 / 02期

关键词：

PROGRAMMED DNA FRAGMENTATION; APOPTOSIS; TNF-ALPHA; CERAMIDE; SPHINGOMYELINASE;

D O I：

10.1016/0014-5793(94)01428-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have found that a solvent mixture of ethanol and dodecane was able to disperse natural ceramide (Cer) into aqueous solution. In U937 cells, addition of natural Cer to medium caused a characteristic pattern of DNA fragmentation, which is indistinguishable from that caused by TNF alpha. The effective concentration of Cer is as low as 25 nM. The present study provides direct evidence that natural Cer functions as a second messenger mediating TNF alpha-induced DNA fragmentation. The use of this solvent to deliver hydrophobic natural Cer to cells mill contribute to the elucidation of the biological function of Cer.

引用

页码：211 / 214

页数：4

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