RECOVERY OF PROTEINS FROM REVERSED MICELLES USING A NOVEL ION-EXCHANGE MATERIAL

被引：4
作者：
CHAUDHURI, JB
SPIROVSKA, G
机构：
[1] School of Chemical Engineering, University of Bath, Bath, BA2 7AY, Claverton Down
来源：
BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 12期
关键词：
D O I：
10.1007/BF02447738
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The recovery of proteins solubilised inside reversed micelles is generally a low yielding process. We have studied three methods for recovery of lysozyme. The protein laden organic phase was contacted with solutions of varying pH. Greater than 95% recovery was achieved at pH values close to the isoelectric point (10.9). The use of 2.5 M KCI gave close to 100% recovery of lysozyme. The organic phase was passed onto a ion exchange matrix. This resulted in disruption of the reversed micelle releasing 60-80% of the protein into the eluent.
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页码：909 / 914
页数：6
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