ANALYSIS OF COMMON ANTIGEN OF FLAGELLA IN BACILLUS-CEREUS AND BACILLUS-THURINGIENSIS

被引：7
|
作者：
MURAKAMI, T
HIRAOKA, K
MIKAMI, T
MATSUMOTO, T
KATAGIRI, S
SHINAGAWA, K
SUZUKI, M
机构：
[1] TOHOKU COLL PHARMACEUT SCI,DEPT MICROBIOL,4-4-1 KOMATSUSHIMA,AOBA KU,SENDAI,MIYAGI 981,JAPAN
[2] BANYU PHARMACEUT CO LTD,QUAL CONTROL LAB,OOSATO,SAITAMA,JAPAN
[3] YAMAGATA PREFECTURAL PUBL HLTH LABS,YAMAGATA,JAPAN
[4] IWATE UNIV,FAC AGR,DEPT VET MED,MORIOKA,IWATE 020,JAPAN
来源：
FEMS MICROBIOLOGY LETTERS | 1993年 / 107卷 / 2-3期
关键词：
BACILLUS-CEREUS; BACILLUS-THURINGIENSIS; FLAGELLAR COMMON ANTIGEN; MONOCLONAL ANTIBODY;
D O I：
10.1111/j.1574-6968.1993.tb06027.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus. This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot assay. Monoclonal antibody H15A5 against common antigenic epitope of B. cereus also reacted with flagellar antigens of 21 strains of Bacillus thuringiensis by ELISA. This monoclonal antibody reacted with the 61-kDa protein of the flagella of B. cereus H.1 and H.2 and B. thuringiensis Kurstaki HD1, Alesti and Aizawai juroi by immunoblot analysis. These results indicated that the common antigenic epitope of the 61-kDa protein existed in the flagella both of B. cereus and B. thuringiensis.
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页码：179 / 183
页数：5
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