PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST THYROXINE

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DANILOVA, NP

KRUPINA, NV

MERTZ, MV

VASILOV, RG

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BIOORGANICHESKAYA KHIMIYA | 1993年 / 19卷 / 07期

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Spleen cells of BALB/c mouse immunized with the keyhole limpet haemocyanin and thyroxine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells to produce. Four monoclonal antibodies selected by indirect ELISA and partially characterized. One antibody, 1B7, chosen for immunoassay, was produced in mouse ascites fluid, purified and analyzed; it proved to belong to the IgG1 subclass. The cross-reactivity with triiodothyronine was less than 1%, the association constant was 3 . 10(9) M-1.

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页码：704 / 712

页数：9

相关论文

共 8 条

[1] MEASUREMENT OF MONOCLONAL-ANTIBODY AFFINITY BY NONCOMPETITIVE ENZYME-IMMUNOASSAY [J].

BEATTY, JD ;

BEATTY, BG ;

VLAHOS, WG .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 100 (1-2) :173-179

[2] RADIOIMMUNOASSAY OF THYROXINE [J].

CHOPRA, IJ ;

SOLOMON, DH ;

HO, RS .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1971, 33 (05) :865-+

[3] PRODUCTION AND CHARACTERIZATION OF ANTI-THEOPHYLLINE MONOCLONAL-ANTIBODIES SUITABLE FOR IMMUNOASSAY [J].

DANILOVA, ND ;

VASILOV, RG .

IMMUNOLOGY LETTERS, 1991, 28 (01) :79-84

[4] A NOVEL ENZYME-IMMUNOASSAY FOR TOTAL THYROXINE USING IMMOBILIZED ANTIBODIES AND HYDROPHOBIC CHROMATOGRAPHY PURIFIED THYROXINE-PEROXIDASE CONJUGATE [J].

FERRUA, B ;

GENETET, F ;

SAVARON, ML ;

MOULIN, C ;

SALARD, JL ;

MASSEYEFF, R .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 87 (02) :137-143

[5] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

[6] THE PRODUCTION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY TO THYROXINE [J].

MPOKO, CN ;

GORDON, DB ;

LAING, I ;

CORBITT, G ;

STOREY, CC .

CLINICA CHIMICA ACTA, 1985, 146 (2-3) :215-222

[7]

SIEBERT GR, 1989, Patent No. 4888296

[8] MECHANISM OF INHIBITION OF MALATE-DEHYDROGENASE BY THYROXINE DERIVATIVES AND REACTIVATION BY ANTIBODIES - HOMOGENEOUS ENZYME IMMUNOASSAY FOR THRYOXINE [J].

ULLMAN, EF ;

YOSHIDA, RA ;

BLAKEMORE, JI ;

MAGGIO, E ;

LEUTE, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 567 (01) :66-74