ELECTROPHORETIC DETERMINATION OF CARBOXYLESTERASE ACTIVITY IN THE MITOCHONDRIAL-FRACTION OF THE RENAL-CORTEX OF THE GUINEA-PIG

被引：0
作者：
DEBORD, J
COMTE, JH
LACHATRE, G
LAVERGNE, F
机构：
来源：
ANALUSIS | 1993年 / 21卷 / 01期
关键词：
CARBOXYLESTERASE; ELECTROPHORESIS; GUINEA PIG; MITOCHONDRIAL FRACTION; RENAL CORTEX;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Carboxylesterase activity has been determined in the mitochondrial fraction of the guinea pig renal cortex by electrophoresis. Enzymes were stained with 2-naphthyl acetate and quantified by densitometry. Isoelectric focussing separates eight fractions (pI 5.0-5.7). Gradient polyacrylamide gel electrophoresis separates six fractions (M(r) 78 to 280 kDa). Isoelectric focussing could be used to study the action of nephrotoxic compounds on kidney esterases.
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页码：45 / 47
页数：3
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