PURIFICATION AND PROPERTIES OF DEXTRANSUCRASE FROM STREPTOCOCCUS SANGUIS

被引：124

作者：

CARLSSON, J

NEWBRUN, E

KRASSE, B

机构：

[1] Department of Oral Microbiology, University of Lund, School of Dentistry, Malmö

来源：

ARCHIVES OF ORAL BIOLOGY | 1969年 / 14卷 / 05期

关键词：

D O I：

10.1016/0003-9969(69)90140-X

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Dextransucrase from Streptococcus sanguis was isolated from the supernatant fluid of a glucose-containing broth culture. Purification of this constitutive enzyme was achieved in two steps by adsorption on hydroxylapatite followed by iso-electric focusing and fractionation. This resulted in a 400-fold purification of the enzyme which was obtained in about a 15 per cent yield. The enzyme had a pH optimum between 5.2 and 7.0, Michaelis constant of 4.8 mM sucrose, optimal activity at 45 °C and an iso-electric point at pH 7.9. Certain of these properties are of significance for the accumulation of bacteria on the teeth. © 1969.

引用

页码：469 / &

共 30 条

[1]

BAILEY JL, 1967, TECHNIQUES PROTEIN C, P341

[2] IMMUNOPOLYSACCHARIDES .6. THE ISOLATION AND PROPERTIES OF THE DEXTRANSUCRASE OF BETACOCCUS-ARABINOSACEOUS [J].