STORAGE AND IMMOBILIZATION OF PHOTOSYSTEM-II REACTION CENTERS USED IN AN ASSAY FOR HERBICIDES

被引：15

作者：

BREWSTER, JD

LIGHTFIELD, AR

BERMEL, PL

机构：

[1] U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Pennsylvania, 600 East Mermaid Lane, Philadelphia

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 07期

关键词：

D O I：

10.1021/ac00103a024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Significant improvements in a previously described method for detection of herbicides at nanogram per milliliter concentrations are described. The activity of photosystem II reaction centers from spinach thylakoids was extended from hours to over 6 months by rapid freezing and storage at -70 degrees C in appropriate cryoprotection media. Freeze-dried reaction centers were also stable for at least 3 months at 4 degrees C. Reaction centers immobilized in calcium alginate gels retained activity for 1 week at 4 degrees C, and immobilization permitted optical and chemical interference by milli samples to be eliminated.

引用

页码：1296 / 1299

页数：4

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