FLUORESCENCE IMAGING CREATES A WINDOW ON THE CELL

被引：45

作者：

TSIEN, RY ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,HOWARD HUGHES MED INST,SAN DIEGO,CA 92103

来源：

CHEMICAL & ENGINEERING NEWS | 1994年 / 72卷 / 29期

关键词：

D O I：

10.1021/cen-v072n029.p034

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Advances in the design and synthesis of fluorescent molecules that reversibly bind chemical messengers in living cells allow researchers to observe the dynamics of a cell's internal biochemistry. This paper discusses the mechanism of tracking Ca ions in cells, probing of [Ca2+]i in diseases, imaging cyclic adenosine monophosphate (cAMP) in vivo, describes how fluorescence microscopy makes intracellular imaging possible, and describes the design of indicators for biological applications.

引用

页码：34 / 44

页数：11